pCR-Blunt II-TOPO Parental vector for TOPO cloning of blunt PCR products. Fisher Scientific is always working to improve our content for you. Not for use in diagnostic procedures. Zero Blunt TOPO PCR Cloning Kit (Cat.
Invitrogen Zero Blunt TOPO PCR Cloning Kit, without competent cells K2800-20) including One Shot Top10 Chemically Competent E. coli cells (other competent cells for cloning may also be used) Sterilized glass beads (EMD Millipore, cat. Search
Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Not for use in diagnostic procedures. Flexible.
Zero Blunt TOPO PCR Cloning Kit, without competent cells Please use the form below to provide feedback related to the content on this product. We will not share your information for any other purposes. Fisher Scientific is always working to improve our content for you. How can I be notified when a plasmid from a specific lab or paper is available? Thermo Fisher Scientific. The pCRBlunt II-TOPO vector contains: The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. Blunt DNA Cloning Vectors, TOPO Cloning Vectors, pCR, Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems TaqMan Assay and Arrays Search Tool, Applied Biosystems TaqMan Custom Assay Design Tools, Applied Biosystems Custom qPCR Primers and TaqMan Probes Tool, Chemical Storage and Management Resource Center, EcoRI sites flanking the PCR product insertion site for easy excision of inserts, Kanamycin and Zeocin resistance genes for your choice of selection in, SP6 promoter/primer site for in vitro RNA transcription and sequencing, M13 forward and reverse primer sites for sequencing or PCR screening, Fast and easy: go from PCR to clones in just three steps and in as little as 5 minutes hands-on time, Efficient: obtain up to 95% clones with correct insert, Proven: reliable performance for over a decade with over 4,000 citations, Simple: no ligase, post-PCR procedures, or PCR primers containing specific sequences are required, Vector: pCRBlunt II-TOPO vector: contains the, Cloning method: TOPO cloning: Topoisomerase Ibased, 5-minute ligation of blunt-ended PCR products amplified with proofreading thermostable polymerases, Competent cells: Various options: choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing competent cells (available separately) or use your own, Antibiotic resistance: Ampicillin (AmpR), Kanamycin (KanR).
pCR4-TOPO Sequence and Map - SnapGene Explore Over 2.7k Plasmids: I.M.A.G.E. Efficient cleavage requires at least two copies of the RsrII recognition sequence. Please note: Your browser does not support the features used on Addgene's website. Each Zero Blunt TOPO for subcloning Kit contains linearized and topoisomerase I-activated pCR Blunt II-TOPOVector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot Chemically Competent or ElectrocompE. coli, S.O.C. Does Addgene accept orders by fax, phone or email? Systems, Research If you run into any problems registering, depositing, or ordering please contact us at [emailprotected]
By continuing to use this site, you agree to the use of cookies. Receive the latest news, hot plasmids, discounts and more. pCR-Blunt II-TOPO . Le kit de clonage de PCR Zero Blunt sappuie sur le vecteur de clonage multifonctions PCR-Blunt et la ligase dADN ExpressLinkT4 de faon gnrer temprature ambiante et en cinq minutes un produit de ligature. There is a problem with the plasmid I received.
PDF QUICK REFERENCE Zero Blunt TOPO PCR Cloning Kit - Fisher Sci Get the cloning results you can count on Twenty years ago, Invitrogen TOPO technology became known for superior quality and game-changing innovation in cloning. Molecular Genetics TOPO Vector Cloning Vector Human Molecular Genetics Most recent answer Magnus Wohlfahrt Rasmussen University of Copenhagen 11th Apr, 2015 That's true, you can make a regular. Not for use in diagnostic procedures. 15(24):3243-8. . Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases.
Addgene: Lox-Stop-Lox TOPO Editing, Cloning & ORFs.
pCR4Blunt-TOPO (linearized) Sequence and Map - SnapGene The pCRBlunt II-TOPO vector used in this kit comes with lethal ccdB gene for efficient selection of blunt-ended PCR recombinants products generated by thermostable proofreading polymerases. Receive the latest news, hot plasmids, discounts and more. Invitrogen Zero Blunt TOPO technology provides a highly efficient, 5-minute, one-step cloning strategy for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Fisher Scientific is always working to improve our content for you. The pCR-Blunt II-TOPO Vector also features: For Research Use Only. Introduction Follow these instructions to TOPO Clone your blunt-end PCR product into pCR-Blunt II-TOPO and transform the reaction into chemically competent E. coli cells. 2001 Dec 15.
PDF CRISPR/Cas9-Directed Genome Editing of Cultured Cells - Harvard University Simple. Does Addgene accept orders by fax, phone or email? Speed is less than 5000g.
PDF TOPO PCR cloning solutions - Thermo Fisher Scientific For transformation of electrocompetent cells, a diagram of the multiple cloning site, and a manual, see www.lifetechnologies.com/support or contact Technical Support. How do I prepare and deposit my plasmids? This is a free resource for the scientific community that is compiled by Addgene. Please use the form below to provide feedback related to the content on this product. Thermo Fisher Scientific, Save time by having your items shipped automatically. Have questions about your order, deposit, or a plasmid? For Research Use Only. PCR-Blunt II-Topo kit (Invitrogen, cat.
In addition, a Zero Blunt TOPO Cloning Kit is available combined with a Invitrogen PureLink Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis. The pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase. All contact information provided shall also be maintained in accordance with our
PDF Zero Blunt PCR Cloning Kit - Thermo Fisher Scientific | Download SnapGene Viewer. Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems TaqMan Assay and Arrays Search Tool, Applied Biosystems TaqMan Custom Assay Design Tools, Applied Biosystems Custom qPCR Primers and TaqMan Probes Tool, Chemical Storage and Management Resource Center, EcoRI sites flanking the PCR product insertion site for easy excision of inserts, Kanamycin and Zeocin resistance genes for your choice of selection in, SP6 promoter/primer site for in vitro RNA transcription and sequencing, M13 forward and reverse primer sites for sequencing or PCR screening, Fast and easy: go from PCR to clones in just three steps and in as little as 5 minutes hands-on time, Efficient: obtain up to 95% clones with correct insert, Proven: reliable performance for over a decade with over 4,000 citations, Simple: no ligase, post-PCR procedures, or PCR primers containing specific sequences are required, Vector: pCRBlunt II-TOPO vector: contains the, Cloning method: TOPO cloning: Topoisomerase Ibased, 5-minute ligation of blunt-ended PCR products amplified with proofreading thermostable polymerases, Competent cells: Various options: choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing competent cells (available separately) or use your own, Antibiotic resistance: Ampicillin (AmpR), Kanamycin (KanR). Consortium Plasmids Source/Author: Invitrogen (Life Technologies) Selection Marker: Neomycin/G418 (Geneticin) Copy Number: What is virus associated DNA, and why do I have to order it. Les kits de clonage par PCR Zero Blunt contiennent: un vecteur PCR-Blunt linaris, une ligase dADN T4 ExpressLink, 5X tampons de ligase dADN T4 ExpressLink, un modle de contrle, des dNTPs, de leau strile et des amorces directes et inverses M13. no. pCRBlunt II-TOPO vector: designed for proofreading polymerasesThe pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase.
Addgene - Analyze Sequence TOPO Blunt-End for Subcloning | Thermo Fisher Scientific - CN ("TOPO" Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector. Not for use in diagnostic procedures. pCR-Blunt II- K n TOPO in 3519 bp site: 1099-1236 bases gene bases sit ORF:586-888: bases406-425 bases 433-448 1237-2031 Zeocin
These products typically do not have pictures or detailed descriptions. & ORFs. Today, Invitrogen TOPO PCR cloning kits are still among the fastest, simplest, and most flexible cloning solutions available. Kit includes linearized and topoisomerase I-activated pCR-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase. Subscribe. We will not share your information for any other purposes. However, we are committed to improving your shopping experience. Efficient cleavage requires at least two copies of the NaeI recognition sequence. Privacy Policy. How It Works The plasmid vector (pCRII-TOPO or pCR2.1-TOPO) is supplied linearized with: Single 3 thymidine (T) overhangs for TA Cloning 450245) is shipped with only the Zero Blunt TOPO PCR Cloning reagents (Box 1). Genome
Linearized vector with bound topoisomerase, for TOPO cloning of blunt PCR products. However, we are committed to improving your shopping experience. We appreciate your feedback. The - pCR-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZ (Bernard et al., 1994).Ligation of a blunt PCR fragment disrupts expression Exceptional. Backbone manufacturer. M13 forward and reverse primer sites for sequencing or . 11. Les kits cellulaires comptents contiennent des cellules d, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Colonnes et cartouches de chromatographie, Consommables en plastique et fournitures de laboratoire, Afficher toutes les catgories de produits, Spectroscopie, analyse lmentaire et isotopique, Voir toutes les applications et techniques, Services aux organisations de dveloppement et de fabrication sous contrat (CDMO) et pour les essais cliniques, Consultez toutes les rubriques d'aide et d'assistance. Resistant to Kanamycin (KanR) and Zeocin (ZeoR). Each kit has the Zero Blunt TOPO vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants so you can obtain up to 95% clones with correct insert. You may not be able to create an account or request plasmids through this website until you upgrade your browser.
By continuing to use this site, you agree to the use of cookies. & Engineering, Model 4. I have used 4 ul of salt.
pCR-Blunt II-TOPO Benchling Plasmid Lox-Stop-Lox TOPO from Dr. Tyler Jacks's lab contains the insert loxP-STOP-loxP and is published in Genes Dev. Includes the Zero Blunt TOPO vector containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants. These products typically do not have pictures or detailed descriptions. Please note: Your browser does not support the features used on Addgene's website. This is a free resource for the scientific community that is compiled by Addgene. The maps, notes, and annotations in the zip file on this page are copyrighted material. I have used 1 ul of 10 ng/ul insert and 1 ul of TOPO vector.
Addgene: gRNA_GFP-T1 Has anyone grown pCR-BluntII Topo in-house? | ResearchGate How do I prepare and deposit my plasmids? Sign Up for Our Newsletter. Search Resistant to Kanamycin (KanR) and Zeocin (ZeoR). The pCRBlunt II-TOPO vector contains: EcoRI sites flanking the PCR product insertion site for easy excision of inserts How can I track requests for my plasmids? Each kit has the Zero Blunt TOPO vector (see figure) containing the, Blunt DNA Cloning Vectors, TOPO Cloning Vectors, pCR, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Zero Blunt TOPO PCR Cloning Kit, without competent cells, Spectroscopy, Elemental and Isotope Analysis. Please use the form below to provide feedback related to the content on this product. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. The pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase. The pCR Blunt II-TOPO vector features: Contains the ccdB gene for positive selection; EcoRI sites flanking the PCR product insertion site for easy excision of inserts; Kanamycin and Invitrogen Zeocin resistance genes for your choice of selection in E. coli; SP6 promoter/primer site for in vitro RNA transcription and sequencing; M13 forward and reverse primer sites for sequencing or PCR screening Two vials of lyophilized plasmid will Contact Addgene. Not for use in diagnostic procedures. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. Consortium Plasmids|More Plasmid Sets No matches HomePlasmidsI.M.A.G.E. no. Close the tube and incubate for 10 minutes at room temperature. Learn more, Please note: Your browser does not fully support some of the features used on Addgene's website. pCR-Blunt II-TOPO (linearized) Linearized vector for TOPO cloning of blunt PCR products. SP6 promoter/primer site for in vitro RNA transcription and sequencing. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. & Engineering, Model Zero Blunt TOPO PCR Cloning Kit supplied with the PureLink Quick Plasmid Miniprep Kit (Cat.
Therapeutic homology-independent targeted integration in retina and Carefully open the tube and add 20 l of sterile water to dissolve the DNA. Thermo Fisher Scientific, Zero Blunt TOPO PCR Cloning Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO Cloning") for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Privacy Policy. |, Publications These products typically do not have pictures or detailed descriptions. The pCRBlunt II-TOPO vector used in this kit comes with lethal ccdB gene for efficient selection of blunt-ended PCR recombinants products generated by thermostable proofreading polymerases. This website uses cookies to ensure you get the best experience. The Zero Blunt TOPO PCR Cloning Kit for Subcloning, without competent cells allows the flexibility of using your own strain for increased flexibility so competent cells and S.O.C. Please sign in to view account pricing and product availability. For Research Use Only. We appreciate your feedback. What is an MTA/Who is authorized to sign? Genome Try SnapGene for Free. How do I place an order? SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. 1333 Xinlong Road, Minhang District, Shanghai, China, Zip-code: 201101, Copyright 2014 NovoPro Bioscience Inc. All rights reserved. You may not be able to create an account or request plasmids through this website until you upgrade your browser. What strain of bacteria does my stab contain? The standard in cloning When it comes to cloning, TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form.
Addgene: Vector Database - pCR blunt II TOPO Try SnapGene for Free.
Invitrogen Zero Blunt TOPO PCR Cloning Kit, without competent cells Download Plasmid. Citing NovoPro. The pCR Blunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum SuperFi DNA Polymerase. This vector is NOT available from Addgene. Learn about the latest plasmid technologies and research tools. Fast accurate construct design for all major molecular cloning techniques, Validate sequenced constructs using powerful alignment tools, Customize plasmid maps with flexible annotation and visualization controls, Automatically generate a rich graphical history of every edit and procedure, Gain unparalleled visibility of your plasmids, DNA and protein sequences, Annotate features on your plasmids using the curated feature database, Store, search, and share your sequences, files and maps.
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